Actinoallomurus acanthiterrae sp. nov., an actinomycete isolated from rhizosphere soil of the mangrove plant Acanthus ilicifolius

Abstract

A novel actinobacterium strain, 2614A723(T), was isolated from rhizosphere soil of mangrove plant Acanthus ilicifolius collected at Touyuan, Wenchang, Hainan province, China. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 2614A723(T) formed a distinct phyletic line in the genus Actinoallomurus, the 16S rRNA gene tree sharing similarities of 98.35%, 98.07% and 97.86% with Actinoallomurus spadix NBRC 14099(T), Actinoallomurus purpureus TTN02-30(T) and Actinoallomurus luridus TT02-15(T), respectively. Strain 2614A723(T) contained lysine and meso-diaminopimelic acid in the cell wall peptidoglycan and madurose, galactose and xylose in the whole-cell sugars. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major polar phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The predominant fatty acids were iso-C16 : 0 and anteiso-C17 : 0. These chemotaxonomic data confirmed the affiliation of strain 2614A723(T) to the genus Actinoallomurus. It is apparent from the combined phenotypic data, biochemical tests and DNA-DNA hybridization values that strain 2614A723(T) should be classified in the genus Actinoallomurus as a representative of a novel species. The name Actinoallomurus acanthiterrae sp. nov. is proposed with strain 2614A723(T) ( = CCTCC AA 2012001(T) = DSM 45727(T)) as the type strain.