Abstract
Chloroplast movement is regulated by dynamic reorganization of the actin cytoskeleton. To gain insight into the function of ACT2 in chloroplast movement, we examined the effect of the act2-3 mutation, in which a T-DNA is inserted at the second exon of the ACT2 gene, and investigated a transgenic Arabidopsis plant expressing the GFP-ACT2 fusion protein. Chloroplast movement in the act2-3 mutant was retarded, especially during the avoidance response, compared with that in the WT. We further verified the physiological response of transgenic Arabidopsis expressing the GFP-ACT2 fusion gene with a 6xGSS linker sequence, which was recently reported to enable the direct visualization of actin filaments in a transient expression system. Although the expression of GFP-ACT2 had highly detrimental effects on the growth and development of transgenic Arabidopsis plants, GFP-ACT2 was incorporated into chloroplast actin (cp-actin) filaments instead of cortical actin filaments. The analyses of the act2 mutant and GFP-ACT2 transgenic plants suggest that ACT2 functions in chloroplast movement as a component of cp-actin filaments and that cp-actin filaments are differently regulated compared to cortical actin filaments.
Published Version
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