Abstract
The yeast Saccharomyces cerevisiae, has proven to be a powerful model organism in which to study actin function and structure-function relationships. This yeast has a single actin gene, a feature that greatly simplifies molecular-genetic and biochemical analysis of actin. Yeast actin is 88% identical to mammalian actin and its biochemical properties are similar to all other actins studied (Nefsky and Bretscher 1992). The ease of molecular genetics in yeast has allowed the generation of numerous site-specific actin mutants. Furthermore, the nonlethal actin mutants have been expressed in yeast as the sole source of actin (Shortle et al. 1984), allowing elucidation of biological importance via phenotypic analysis, and purification and biochemical characterization of mutant actins.KeywordsActin FilamentActin PatchActomyosin InteractionActin MutantYeast ActinThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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