Actin bundles in perineurial cells of rat spinal nerves.

Abstract

The overall distribution of the actin cytoskeleton in perineurial cells of rat spinal nerves was examined by confocal laser and thin-section electron microscopy. Confocal laser microscopy of whole-mount nerves stained with fluorescent-labelled phalloidin revealed two types of actin bundles in perineurial cells; stress fiber-type actin bundles and circumferential actin bundles. The degree of development of the actin cytoskeleton varied in different segments of different nerves. Stress fiber-type actin bundles were also immunostained for myosin and vinculin and were well-developed in the perineurial cells of large-sized nerves and dorsal root ganglia, whereas they were poor in spinal nerve root sheaths within the subarachnoid space. In peripheral nerves, stress fiber-type actin bundles tended to be arranged transverse to the nerve axis. Circumferential actin bundles were localized along intercellular junctions, which were immunostained with several junctional proteins such as alpha-catenin, occludin and ZO-1. Thin-section electron microscopy confirmed the distribution pattern of actin bundles observed by confocal laser microscopy. These findings suggest that actin bundles may play some roles in structurally stabilizing the perineurium by providing mechanical support for the cell layers as well as cell junctions to maintain perineurial integrity and form diffusion barriers in peripheral nerves.