Abstract
Using rats pretreated with a preparation of adrenal cortical extract (Adrex “Sankyo”) so as to suppress the secretion of ACTH from cerebral hypophysis, it was examined whether this could be used for the assay of ACTH. Sixteen hours after subcutaneous injection of Adrex, corresponding to 80g. of adrenal, with 2mg. of ascorbic acid, the rat was anesthetized with pentobarbital, its left adrenal taken out, and the test solution was injected intravenously. After 1 hour, its right adrenal was taken out and the ascorbic acid concentration in the two adrenals was compared. The difference value itself and the decreased rate were taken as the respective responses and analyzed statistically. In 0.625-5.0γ/100g. dose, lineality of the log dose-response curve was observed but the value λ was found to be too large and varied.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
