Act of Malondialdehyde and Total Phenolic Content Under Bean Yellow Mosaic Virus Infection and Biostimulants Application

Karar Hamzah,Ahmed Abdelkhalek,Hosny Younes,Said Behiry

doi:10.21608/eajbsh.2021.148885

Abstract

Bean yellow mosaic virus represents a stress effect on fava bean plants. This study was conducted to assess the lipid peroxidation and total phenolic content (TPC) of fava bean plants under bean yellow mosaic virus infection and its management by the biostimulants; Rhizobium and Bacillus species spraying application. The obtained data revealed that virus-infected plants maximized stress factors, the content of malondialdehyde (MDA), while it decreased in other applications. TPC significantly increased in Bacillus, and Rhizobium isolates spraying treatments with 20.99 and 22.44 mg/100g extract, respectively, compared with control. The current work provides bio-resistance by Bacillus or Rhizobium isolates effectively minimizes the adverse effects of challenges such as stressing viral infection and representing an environmentally friendly commercial value for plant growers.

Highlights

Fava bean, Vicia faba L, is one of the oldest crops grown and used as a protein source for humans and animals (Bishnoi et al, 2012)
Bean yellow mosaic virus (BYMV) is the most common and prevalent virus among fava bean viruses (Sofy et al, 2019). 89% of the Egyptian fava bean fields surveyed with high-level BYMV symptoms (80–100% infection) (Makouk et al, 2003)
Virus Isolate and Treatments: In this study, bean yellow mosaic virus isolate was used to inoculate the fava bean plants and biostimulants as Bacillus and Rhizobium bacterial isolates were sprayed to manage the virus infection, and all treatments illustrated in Table (1) Determination of Lipid Peroxidation: Lipid peroxidation was measured as malondialdehyde (MDA) content, a thiobarbituric acid reactive substance, according to Heath and Packer (1968)

Summary

Introduction

Vicia faba L, is one of the oldest crops grown and used as a protein source for humans and animals (Bishnoi et al, 2012). This crop fixes atmospheric nitrogen in a symbiotic relationship with rhizobium bacteria in the soil (Karla, 2009). It plays a significant dietary role in supplying proteins, carbohydrates, essential elements, and vitamins to rural and urban people. 89% of the Egyptian fava bean fields surveyed with high-level BYMV symptoms (80–100% infection) (Makouk et al, 2003) Bean yellow mosaic virus (BYMV) is the most common and prevalent virus among fava bean viruses (Sofy et al, 2019). 89% of the Egyptian fava bean fields surveyed with high-level BYMV symptoms (80–100% infection) (Makouk et al, 2003)

Objectives

Methods

Results