Abstract

When acridine orange is used at 10−5 or 10−6M concentrations in supravital preparations of mouse or grasshopper testicular cells, it is evident that condensed chromatin in spermatogenic cells displays considerably higher levels of fluorescence than extended chromatin in vesicular nuclei. This result differs from that obtained traditionally in appropriately fixed and stained cytological preparations in which fluorescence is greatest in extended chromatin and lowest in condensed chromatin (Rigler, 1966, and others). While an explanation for this reversed staining pattern in unfixed cells is not immediately available, it is clear that acridine orange may offer novel opportunities for the experimental manipulation and examination of the chromosomes (for deoxyribonucleoprotein complex) of living cells.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.