Abstract
A soil microorganism producing iron chelator (siderophore) under low iron stress (up to 2 μM of iron) was identified as Acinetobacter sp. B-W by 16S rDNA sequence analysis, biochemical-, physiological tests and morphological analysis using electron microscope. Catechol nature of siderophore was detected by Arnow test. Although optimal cell growth was identified at 36°C in iron-limited media, significant quantities of siderophore were produced only at 28°C. Biosynthesis of siderophore was strongly inhibited by growth at 36°C. Production of siderophore was completely inhibited by 10 μM FeCl3. Iron chelator produced from Acinetobacter sp. B-W was purified from supernatant using butanol extraction, Sephadex LH-20 column chromatography and HPLC. Purified sideropore was identified as 2,3-dihydroxybenzoic acid by HPLC, TLC and IR analysis.
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