Abstract
Factors influencing the response of Rhizobium strains to acidity and aluminium were studied in an arabinose-galactose-glutamate liquid medium. Rhizobium trifolii strains HP3 and BEL 1192 multiplied at the same rate at pH S.S. BEL1192 multiplied at a slower rate at pH 4.5, and all produced visible turbidity from a low initial cell density. HP3 multiplied at pH 4.5 but produced no turbidity. The pH value of the medium increased when cell densities reached 10 6-10 7 cfu ml −1. Diauxic growth responses occurred, only at pH 4.5, and HP3 demonstrated a population effect at pH 4.5 with visible turbidity produced from high initial cell densities. 50 μm Al caused a decrease in numbers of BEL 1192 at pH4.5 and HP3 at pH 5.5 irrespective of initial cell densities. 50 μ m Al was more toxic to HP3 cells in log phase than in stationary phase.
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