Acidification of testicular and epididymal fluids in the rat after surgically-induced varicocele.

Abstract

Experimental left varicocele (ELV) in adult rats produces a bilateral increase in testicular blood-flow and temperature which may alter the intraluminal environment in which sperm mature. The purpose of the present study was to evaluate the effect of ELV on the in-situ pH, PCO2 and bicarbonate concentration ([HCO3-]) in seminiferous tubules (ST), initial segment (IS), proximal caput (PCP), middle caput (MCP), middle corpus (MCR), and proximal cauda epididymidis (PCD) of the rat employing pH and PCO2 microelectrodes. Adult male rats with ELV or sham-surgeries (control) were studied after 30 days. Relative to controls, ELV significantly reduced the in-situ PCO2 in the testicular artery, ST, IS, PCP, MCP, MCR and PCD. In spite of this reduction, all values remained significantly higher than the systemic arterial blood PCO2. Values for in-situ pH in ST, IS, PCP, MCP, MCR, and PCD of control rats were significantly more acidic than systemic arterial blood. Furthermore, ELV decreased the pH in ST, IS, PCP, and MCP when compared to control values. The calculated [HCO3-] in ST from control animals was less than half that in systemic arterial blood and was reduced further in IS, PCP, and MCP. Varicocele did not change the [HCO3-] in systemic arterial blood but reduced markedly the values in ST, IS, PCP, and MCP. These alterations in the in-situ pH, PCO2, and [HCO3-] in structures of the rat testis and epididymis may play a role in the anti-spermatic effect of ELV.