Abstract
Acidic and neutral oils in Haccho Miso were obtained by ether extraction, acid and alkali treatments.The acidic oil was separated further by steam distillation, and the volatile fraction was fractionated into 22 fractions, the none volatile fraction which was treated with diazomethane was fractioneted into five fractions by gaschromatography.Seventeen compounds in these were identified as acetic, propionic, iso-butylic, n-butylic isovaleric, n-valeric, iso-caproic, n-caproic, enanthic, caprylic, pelargonic, capric, palmitic, stearic, oleic, linolic and linolenic acid.The high boiling fraction of neutral oil was separated by steam distillation. Ten compounds were identified from the high boiling fraction.There compounds were as palmitic, stearic, oleic, linolic and linolenic acid methyl ester and ethyl ester, respectively.
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