Abstract
The study aimed to compare differences of physiological acid-base balance (ABB) parameters in follicular fluid (FF) and venous blood (VB) and to evaluate ABB parameters in FF collected from different ovarian follicles in dairy cows and heifers. The ABB parameters (pH, pCO2, pO2, HCO3– and base excess (BE)) in the FF of the preovulatory follicle, of the dominant follicle on the 9th day of the cycle and of the superovulatory estrous follicles were compared to VB. Similarly, the dynamics of the ABB profile in FF and VB were monitored in repeated sampling in a group of heifers stimulated by follicle-stimulating hormone (FSH). Higher values of pH and pO2 and lower values of pCO2, HCO3– and BE were found in FF compared to VB in all experiments. Laterality of ovaries, time of sampling, ovarian activity or stimulation of the follicular development by FSH did not significantly influence ABB parameters. We found higher pH (7.392 ± 0.027 vs. 7.364 ± 0.032) and pO2 (13.83 ± 2.20 kPa vs. 4.50 ± 0.67 kPa), lower pCO2 (5.70 ± 0.39 kPa vs. 6.54 ± 0.61 kPa), HCO3– (25.51 ± 1.52 mmol/l vs. 26.86 ± 2.12 mmol/l) and BE (1.14 ± 1.57 mmol/l vs. 1.95 ± 2.2 mmol/l) in FF compared to VB in all non-stimulated cows. Similar relationships between FF and VB were found in all FSH stimulated cows. The study provides as yet unknown knowledge on the physiology of follicular fluid in cattle.
Highlights
Lower Laterality of ovaries, time of sampling, ovarian activity or stimulation of the follicular development by follicle-stimulating hormone (FSH) did not significantly influence acid-base balance (ABB) parameters
The dynamics of the ABB profile in Follicular fluid (FF) and venous blood (VB) were monitored in repeated sampling in a group of heifers values stimulated by follicle-stimulating hormone of pCO2, HCO3– and BE were found in FF
VB and to evaluate ABB indices in FF collected from follicles of different origin in dairy cows higher pO2, and heifers
Summary
Lower Laterality of ovaries, time of sampling, ovarian activity or stimulation of the follicular development by FSH did not significantly influence ABB parameters. Follicular fluid plays a major role in the autocrine and paracrine regulation and in the physiological, biochemical and metabolic aspects of the nuclear and cytoplasmatic maturation of the oocyte and the process of ovulation (Hafez 2000). The relatively constant hydrogen ion concentration [H+] is the result of a balance between acids and bases. Acids or bases are added continuously to the body fluids, either because of their ingestion or as a result of their production in cellular metabolism. Physiological ABB parameters of venous blood (VB) of dairy cattle are well known: 7.38–7.43; 5.2–6.4 kPa; -0.5–4.5 and HCO3–, respectively (Pechova et al.23.5–27 mmol/l for pH, pCO2, base excess (BE) 2015). Several articles in human medicine have dealt with these factors (Shalgi et al 1972; Daya 1988; Imoedemhe et al 1993); they have focused only on concentrations of carbon dioxide and pH in FF
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.