Abstract
Abstract Javanese goat (Capra aegagrus hocus) is an animal species which have many populations in Indonesia and it is used because more selective in feed, especially Leguminosae and seeds which of rich in collagen. The study aims to determine the impact of hydrolysis time and antioxidant activities on the extraction of skin Javanese goats using the acid-soluble collagen (ASC) method. The ASC used in this investigation was taken from another study and hydrolyzed with pepsin throughout the course of 0, 30, 60, 90, and 120 minutes of incubation. The success of the hydrolysis process can be shown by the percent degree of hydrolysis. Methods are used to determine the percent degree of hydrolysis of the TCA method. Results showed that hydrolyzing for 60 min yields the highest total soluble collagen of ASC, with a value of 0.77 mg/ml. Finally, the ASC which is hydrolysis use pepsin for 60 min has a degree of hydrolysis, and total soluble collagen is the highest. The protein molecular weight of ASC which is the hydrolysis of 60 min is smaller than before hydrolysis and it has radical scavenging activity of 56.42% ± 1.45. So far, ASC from Javanese goat (Capra aegagrus hocus) has been hydrolyzed in this study has high antioxidant activity and can be recommended as a substitute for functional food.
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