Abstract
A protease has been isolated from germinated sorghum seeds in a highly purified, crystalline form. A molecular weight of about 80 000 has been obtained for the enzyme from gel filtration experiments. The protease does not require serine or cysteine (sulfhydryl) at the active site and has no requirement for metal ions. This enzyme has been classified as an “acid protease”, since it has its pH optimum at acidic pH range (pH 3.6). Effects of enzyme concentration and substrate concentration on the rate of catalysis have been studied and an apparent Km of 0.76 mM and 0.19 mM has been obtained against bovine serum albumin and N,N‐dimethyl albumin as substrates, respectively.
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