Abstract
Twenty-seven strains of Lotus rhizobia were tested for acid tolerance in yeast-extract mannitol broth (pH 4.6) by multiplication from low initial cell densities. Acid production on unbuffered yeast-extract mannitol agar slopes incorporating bromothymol blue indicator was also determined. All slow-growing, alkali-producing strains were acid sensitive and four of the six fast-growing, acid-producing strains were acid tolerant as was one fast-growing, alkali-producing strain. The method of testing for acid tolerance proved suitable for fast-growing strains and results are discussed in relation to the ecological implications of acid and alkali production by rhizobia.
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