Abstract

AbstractSophorolipids (SL) prepared from biotransformation of refined bleached deodorized (RBD) palm olein by Starmerella bombicola (ATCC 22214) occur as a mixture of homologs. Characterization of SL was performed using high‐performance liquid chromatography with electrospray ionization mass spectrometry detection (HPLC‐ESI‐MS) under negative ion mode. Two different purification methods were compared: precipitation and solvent extraction. SL purified by precipitation and solvent extraction consisted of five major homologs with m/z values of 687, 645, 689, 705, and 661. The fractions of lactone and acidic SL via precipitation were 70.24 ± 1.4% and 29.76 ± 1.4%, respectively. SL isolated by solvent extraction contained 44.78 ± 1.9% lactone and 55.22 ± 1.9% acidic SL. Therefore, the purification method strongly impacted the composition of the SL products.

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