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Abstract
Ethanol producing from biomass has become an interesting substitute to petrolium in the struggle against raised levels of fossil carbon dioxide emission to the atmosphere. The biotechnological conversion of biomass into fuels requires hydrolysis of the polysaccharide fraction into monomeric sugars. This study was aimed at the optimization of the processing conditions of the hydrolysis step of bio-ethanol production to produce high fermentable sugar. In this study dilute acid hydrolysis, temprature and residunce time were used as process parameters. Response SurfaceMethodology (RSM) was employed for the optimization of hydrolysis conditions. A 3 (three) level design was used to develop a statistical model for the optimization of process variables. The raw material, <i>P. juliflra</i> stem, was collected from Afar region eastern part of Ethiopia specifically Wrer Agricultural Research Center. The optimal hydrolysis conditions that resulting for the maximum total reducing sugar concentration were at acid concentration; 2% (v/v), temperature; 128.01°C and hydrolysis time; 55 minutes. Under these conditions, the total reducing sugar concentration was obtained to be 184.716mg/g.
Highlights
IntroductionFuels obtained from renewable resources have deserved a great deal of interest during the past decades mainly due to concerns about fossil fuels depletion and environment pollution
The growing pressure on reduction in the net emission of CO2, global warming, and the use of food crops for ethanol production resulted in global food crises have maintained research and development on the ethanol production from non edible renewable raw materials (Himmel, 2007)
The chemical components for P. juliflora are comparable to other biomass source used for the same objective, for instance, for Eucalyptus grandis residue 40%, 16% and 23% for cellulose, hemicelluloses and insoluble lignin, respectively Canettieri et al, (2007); loblolly pine showed that 43.6%, 21.2% and 26.8% cellulose, hemicelluloses and insoluble lignin, respectively (Puneet et al, 2009)
Summary
Fuels obtained from renewable resources have deserved a great deal of interest during the past decades mainly due to concerns about fossil fuels depletion and environment pollution. Research efforts have been multiplied in the last years as a consequence of constant increasing costs and environmental impact derived from the use of crude-based fuels (Gray & Emptage, 2006). Production of fuel from renewable resources has advantages over fossil fuel in that less environmental impact and sustainability of resource. The growing pressure on reduction in the net emission of CO2, global warming, and the use of food crops for ethanol production resulted in global food crises have maintained research and development on the ethanol production from non edible renewable raw materials (Himmel, 2007). Much progress has been made in many areas of biomass biorefinery, efficient production of cellulosic ethanol from plant biomass remains a challenge
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