Abstract
The methyl green method of DNase assay is applicable in the acid range as well as at pH 7.5. As compared to the DNA degradation product assay, it has the advantages of greater technical simplicity, sensitivity, versatility, and economy of materials. It measures directly the enzymic degradation of DNA and is independent of secondary enzyme effects, such as of nucleotidases and phosphatases, which may influence the rate of formation of acid soluble products. Advantages of the methyl green method compared to other assay methods have been previously discussed.
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