Acetylsalicylic acid supplementation improves protein utilization efficiency while vitamin E supplementation reduces markers of the inflammatory response in weaned pigs challenged with enterotoxigenic E. coli.

Jae Cheol Kim,John L Black,Bruce P Mullan,Robert J E Hewitt,John R Pluske,Robert J Van Barneveld

doi:10.1186/s40104-016-0118-4

Jae Cheol Kim, John L Black + Show 4 more

Open Access

https://doi.org/10.1186/s40104-016-0118-4

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Abstract

BackgroundThis experiment was conducted to test the hypothesis that vitamin E (Vit E) and acetylsalicylic acid (ASA), a cyclooxygenase-2 (COX-2) inhibitor, will additively reduce the production of the immunosuppressive molecule prostaglandin E2 (PGE2) and hence reduce inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli.MethodsThe experiment was conducted in a research facility with 192 individually-housed male weaner pigs (Landrace × Large White) weighing 6.6 ± 0.04 kg (mean ± SEM). The pigs were experimentally infected with an enterotoxigenic strain of E. coli and were allocated to a 2 × 3 factorial design with the respective factors being without and with 125 ppm ASA and three levels of Vit E supplementation (50, 100 or 200 IU/kg diet, dl-α-tocopheryl acetate).ResultsAcetylsalicylic acid supplementation improved average daily gain (P < 0.05) and tended to improve feed:gain ratio (P < 0.10) during the first 14 d after weaning. Acetylsalicylic acid supplementation also improved (P < 0.001) amino acid utilization efficiency (as assessed by plasma urea level) and tended to decrease (P < 0.10) PGE2 production in the liver without affecting small intestinal histology and tight junction protein mRNA expression in the jejunal epithelium. Vitamin E supplementation greater than 100 IU/kg diet sustained both the plasma Vit E concentration (P < 0.001) and plasma haptoglobin content (P < 0.001) after weaning. However, there was no additive effects of the combined supplementation of ASA and Vit E on performance, intestinal barrier function and inflammatory responses of weaned pigs.ConclusionsAlthough ASA and vitamin E improved amino acid utilization efficiency and reduced acute inflammatory responses, ASA and vitamin E did not additively reduce production of PGE2 and inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli.

Highlights

This experiment was conducted to test the hypothesis that vitamin E (Vit E) and acetylsalicylic acid (ASA), a cyclooxygenase-2 (COX-2) inhibitor, will additively reduce the production of the immunosuppressive molecule prostaglandin E2 (PGE2) and reduce inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli
Likoff et al [9] demonstrated a significant additive effect of Vit E (300 IU/kg diet) and ASA supplementation on depression of PGE2 production and mortality in E. coli (LD50)-infected broilers. Such a study to examine the additive effects of ASA and Vit E supplementation has not been conducted in weaner pigs using experimental infection with an enterotoxigenic strain of E. coli. This experiment examined the hypothesis that supplementation of Vit E and ASA, a COX-2 inhibitor, will additively reduce production of PGE2 and reduce the inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli
This study tested the hypothesis that Vit E supplementation and ASA, a COX-2 inhibitor, would additively reduce the production of PGE2 and reduce the inflammatory responses in weaner pigs experimentally infected with enterotoxigenic strain of E. coli (ETEC) in a research facility

Summary

Introduction

This experiment was conducted to test the hypothesis that vitamin E (Vit E) and acetylsalicylic acid (ASA), a cyclooxygenase-2 (COX-2) inhibitor, will additively reduce the production of the immunosuppressive molecule prostaglandin E2 (PGE2) and reduce inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli. Likoff et al [9] demonstrated a significant additive effect of Vit E (300 IU/kg diet) and ASA (intraperitoneal injection of 50 mg/kg body weight) supplementation on depression of PGE2 production and mortality in E. coli (LD50)-infected broilers Such a study to examine the additive effects of ASA and Vit E supplementation has not been conducted in weaner pigs using experimental infection with an enterotoxigenic strain of E. coli. This experiment examined the hypothesis that supplementation of Vit E and ASA, a COX-2 inhibitor, will additively reduce production of PGE2 and reduce the inflammatory responses in weaner pigs experimentally infected with an enterotoxigenic strain of E. coli

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Discussion

Conclusion