Abstract
An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the biosensor: acetylcholinesterase/chitosan-transition metal carbides/graphene/glassy carbon electrode. With the joint modification of graphene and transition metal carbides, the biosensor has a good performance in detecting dichlorvos with a linear relationship from 11.31 μM to 22.6 nM and the limit of detection was 14.45 nM. Under the premise of parameter optimization, the biosensor showed a good catalytic performance for acetylcholine. Compared to the biosensors without modification, it expressed a better catalytic performance due to the excellent electrical properties, biocompatibility and high specific surface area of graphene, transition metal carbides. Finally, the biosensor exhibits good stability, which can be stored at room temperature for one month without significant performance degradation, and has practical potential for sample testing.
Highlights
With the continuous development of the global economy and population, the demand for foods such as vegetables and fruits are increasing
In order to meet the growing demand for food, especially vegetables, and resolve the problem of insects living with the crop, the using of pesticides in agriculture has become widespread in the past few decades
Dichlorvos (DDVP) was used as the representative of organophosphorus pesticides (OPs), and GR was used as the electrode modification material, Ti3C2Tx-CS was used as the immobilization matrix of the enzyme and an electrochemical biosensor was fabricated with structure of AChE/Ti3C2TxCS/GR/glassy carbon electrode (GCE)(Fig 1)
Summary
With the continuous development of the global economy and population, the demand for foods such as vegetables and fruits are increasing. Dichlorvos (DDVP) was used as the representative of OPs, and GR was used as the electrode modification material, Ti3C2Tx-CS was used as the immobilization matrix of the enzyme and an electrochemical biosensor was fabricated with structure of AChE/Ti3C2TxCS/GR/glassy carbon electrode (GCE)(Fig 1).
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