Abstract
A novel acetylcholinesterase (AChE) biosensor based on transition metal carbides (MXenes) nanosheets and chitosan (CS) had been fabricated successfully for detecting organophosphorous pesticides (OPs). The electrochemical behaviors of the AChE/CS-Ti3C2Tx/GCE biosensor were studied by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The parameters affecting the AChE biosensor performance including pH, enzyme loading, concentration of Ti3C2Tx, and inhibition time were optimized. Under optimum conditions, the AChE/CS-Ti3C2Tx/GCE biosensor exhibited good performance for the detection of malathion with a linearity in range of 1×10−14–1×10−8M and the detection limit was found to be 0.3×10−14M. Due to the excellent biocompatible, no-toxicity, film forming ability of chitosan and the high electrical conductivity and large surface area of the Ti3C2Tx nanosheets, the developed biosensor showed acceptable reproducibility, satisfactory stability, and anti-interference ability. Moreover, the analytical performance of the developed biosensor was demonstrated detecting OPs in practical samples with a satisfactory recovery.
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