Abstract
This study describes the measurement of bio-electrical signals caused by enzymatic inhibition of acetylcholinesterase (AChE) for the detection of organophosphorous and carbamate pesticides which are the strong inhibitors of AChE and prevents its normal function of the rapid removal of acetylcholine (Ach). Biosensor Toxicity Analyzer (BTA) was used for the testing and enzyme activity was determined by acetylthiocholine chloride (ATCCl) as enzyme substrate. The monitoring of changes in bio-electrical signals caused by the interaction of biological substances and residues were evaluated. Two samples of cotton were analyzed. Cryogenic homogenization was carried out for sample pretreatment and Soxhlet extraction method (SOX) was used for extraction. The resulted extracts were concentrated and then injected in the BTA. The method shows reasonable results and can successfully be utilized for the detection of residual pesticides on different types of cotton.
Highlights
Cotton has been part of the fabric of human existence for thousands of years
This study describes the measurement of bio-electrical signals caused by enzymatic inhibition of acetylcholinesterase (AChE) for the detection of organophosphorous and carbamate pesticides which are the strong inhibitors of AChE and prevents its normal function of the rapid removal of acetylcholine (Ach)
Electrochemical biosensors for measurement of these pesticides are based on the inhibition of AChE and the inhibition degree is proportional to the pesticide concentration [14]. We report another method for the determination of Organophosphorous and Carbamates pesticides based on acetylcholinesterase inhibition using AC1.W2.R1/ACCHE sensors with the help of Biosensor Toxicity Analyzer (BTA)
Summary
Cotton has been part of the fabric of human existence for thousands of years. Cotton produces the natural fibers used in textiles and clothing and yields a high grade vegetable oil [1]. Direct or primary exposure normally occurs during the application of these compounds and indirect or secondary exposure can take place through the environment or the ingestion of food [4] This is why development of natural biological methods of insect control was initiated. We report another method for the determination of Organophosphorous and Carbamates pesticides based on acetylcholinesterase inhibition using AC1.W2.R1/ACCHE sensors with the help of Biosensor Toxicity Analyzer (BTA). Biosensor toxicity analyzer (BTA) works on the above mentioned principle and monitors the activity of the inhibition of AChE with the help of sensors which are equipped with an enzymatic membrane of AChE enzyme which is immobilized It consists of two major parts, one of which is the Microflow unit and the other is Bioanalyzer.
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