Abstract
As measured on both the guinea pig ileum and the frog rectus abdominis muscle, acetylcholine (Ach) is uniformly distributed along the sciatic nerve. Measured on the ileum, about 75 per cent of the Ach activity is found in the soluble portion of the cell, while on the rectus abdominis, the corresponding figure is about 35 per cent. Total Ach equivalents in sciatic nerve are higher when measured on the rectus abdominis muscle than on the ileum. Evidence is presented that the activity measured on the ileum is attributable to either Ach or a very similar substance. It is suggested that sciatic nerve contains a substance other than Ach to which the frog rectus abdominis muscle is especially sensitive.
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