Abstract
Acetylation for selectively blocking the vicinal diols of carbohydrates has successfully been applied to ultrathin sections in electron microscopy. From buffered glutaraldehyde fixed rat colon and jejunum tissues embedded in LR-White, ultrathin sections were cut, treated with hot acetic anhydride in pyridine and then reacted for periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP). The PA-TCH-SP reaction of all the glycoproteins and glycogen involved in the colonic and jejunal epithelia was selectively blocked by prior acetylation, providing a means of substantiating the presence of vicinal diols of carbohydrates in electron microscopy.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.