Abstract
Three monoclonal α-tubulin antibodies YL½ ( Kilmartin et al., 1982 ), 6-11B-1 ( Piperno and Fuller, 1985) and DM1A ( Blose et al., 1984 ) were used in indirect immunofluorescence (IIF) microscopy of the microtubule (MT) cytoskeleton in tobacco ( Nicotiana tabacum) pollen tubes. The majority of pollen tube MTs contain tyrosinated α-tubulin recognized by YL½. Acetylated α-tubulin revealed by 6-11B-1 was detected in the generative cell and in the kinetochore fibers, in polar spindle regions, and in the cell plate of the phragmoplast during generative cell division. In addition, small fragments of acetylated microtubules were seen in the older parts of the pollen tube grown on a taxol medium. The interaction of pollen tube MTs with mAb 6-11B-1 suggested that acetylation of α-tubulin correlates well with the putative arrays of stable MTs.
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