Acetylated Resveratrol and Oxyresveratrol Suppress UVB-Induced MMP-1 Expression in Human Dermal Fibroblasts.

Jae-Eun Lee,Daeun Song,Dongyup Hahn,Yong Chool Boo,Jijeong Oh,Mijeong Lee,Nam Joo Kang

doi:10.3390/antiox10081252

Jae-Eun Lee, Daeun Song + Show 5 more

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https://doi.org/10.3390/antiox10081252

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Abstract

Resveratrol (RES) and oxyresveratrol (OXYRES) are considered and utilized as active ingredients of anti-aging skin cosmetics. However, these compounds are susceptible to oxidative discoloration and unpleasant odor in solutions, limiting their use in cosmetics. Accordingly, RES and OXYRES were chemically modified to acetylated derivatives with enhanced stability, and their anti-aging effect on the skin and detailed molecular mechanism of their acetylated derivatives were investigated. Acetylated RES and OXYRES lost their acetyl group and exerted an inhibitory effect on H2O2-induced ROS levels in human dermal fibroblast (HDF) cells. In addition, RES, OXYRES, and their acetylated derivatives suppressed UVB-induced matrix metalloproteinase (MMP)-1 expression via inhibition of mitogen-activated protein kinases (MAPKs) and Akt/mTOR signaling pathways. Furthermore, RES, OXYRES, and their acetylated derivatives suppressed type I collagen in TPA-treated HDF cells. Collectively, these results suggest the beneficial effects and underlying molecular mechanisms of RES, OXYRES, and their acetylated derivatives for anti- skin aging applications.

Highlights

Published: 5 August 2021Skin aging is a biological phenomenon involving intrinsic and extrinsic pathways and is phenotypically characterized by skin roughness, pigmentation, and decreased elasticity.Intrinsic aging is a process that occurs over time, whereas extrinsic aging is caused by exposure to environmental factors [1]
These results suggested that acetylation of RES and OXYRES could improve their oxidative stability
To more directly demonstrate the anti-aging effects of AcRES and AcOXYRES on the skin, we evaluated the impact of these compounds on the activity of matrix metalloproteinase (MMP)-1, which plays an important role in collagen degradation in the human skin dermis

Summary

Introduction

Skin aging is a biological phenomenon involving intrinsic and extrinsic pathways and is phenotypically characterized by skin roughness, pigmentation, and decreased elasticity. Intrinsic aging is a process that occurs over time, whereas extrinsic aging is caused by exposure to environmental factors [1]. UVB is the most active constituent of solar light It provokes the generation of reactive oxygen species (ROS), impairs the skin’s antioxidant status, and causes damage to DNA and proteins. Both direct and indirect adverse biological effects induced by UVB can lead to photocarcinogenesis and photoaging, which is clinically characterized as wrinkle formation and loss of skin elasticity [3]. UVB-induced degradation of the extracellular matrix (ECM), as well as decreased expression of collagen and increased expression of matrix metalloproteases (MMPs), can contribute to photoaging [4,5]

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