Abstract
We studied the composition of molecular forms of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in normal and streptozotocin-induced diabetic rat retinal pigment epithelium (RPE). Tissues were sequentially extracted with saline (S 1) and saline-detergent buffers (S 2). About a 50% decrease in AChE molecular forms was observed in the diabetic RPE compared to the controls. Approximately 70% of the BChE activity in normal RPE was brought into solution and evenly distributed in S 1 and S 2. Analysis of the fractions from RPE revealed the presence of G A 1, G A 4 and a small proportion of G H 4 BChE forms in S 1; whereas G A 4 and G A 1 molecules predominate in S 2. A 40% decrease in the activity of G A 4 in S 2 was observed in the diabetic RPE. Our results show that diabetes caused a remarkable decrease in the activity of cholinesterases molecular forms in the RPE. This might be related to the alterations observed in diabetic retinopathy.
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