Abstract

Pyrolytic lignin is the water-insoluble fraction of the bio-oil from rapid pyrolysis. The separation of pyrolytic lignin can improve the extraction of monophenols. Studies reveal the efficacy of phenolic aromatic compounds derived from lignin as antioxidants and as antimicrobials. In this study, we used aqueous acetone solutions to improve the fractionation of eucalypt pyrolytic lignin. Experiments to obtain the pyrolytic lignin were carried out based on a central composite factorial design, and the characterizations were performed by GPC, NMR, FTIR, TGA, GC–MS, antioxidant assays, and antibacterial activity analysis. The most soluble fraction resulted in a more homogeneous fraction, concentrating phenolic, carboxylic, and lower molecular weight compounds. The antioxidant activity was mainly attributed to phenolic compounds with methoxy substituents. Pyrolytic lignin fractions also showed antimicrobial action against Staphylococcus aureus and Escherichia coli.

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