Acetic acid production by the newly isolated Pseudomonas sp. CSJ-3

Juan Wang,Jianbin Zhang,Wenjing Tang,Cuiyan Wang,Chenliang Hao,Huijuan Huang

doi:10.1590/0104-6632.20180351s20160500

Juan Wang, Jianbin Zhang + Show 4 more

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https://doi.org/10.1590/0104-6632.20180351s20160500

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Abstract

ABSTRACT A fermentative acetic acid producing strain, named CSJ-3, was isolated from sludge and identified as Pseudomonas sp. on the basis of 16S rRNA gene analysis. The effects of cultivation conditions on the production of acetic acid by Pseudomonas sp. CSJ-3 were investigated in batch fermentation, and the maximum yield of acetic acid reached up to 0.49 % during 30 h cultivation under the optimum growth condition, including fermentation temperature of 37.0 oC, fermentation pH of 4.50, ethanol concentration of 8 %, carbon source (glucose) amount of 10 g/L, and rotation speed of 120 r/min. When ultraviolet (UV) irradiation was used for the mutation in culture medium to improve the yield of acetic acid, the results showed that the yield of acetic acid reached up to 0.59 %. UV and FTIR confirmed that acetic acid was the major fermented product.

Highlights

Acetic acid that can be produced by geological and biological processes in natural environments is an important raw material in the organic chemical industry and is the suitable carbon source for many microorganisms (Sievers et al, 1992; Trcek et al, 1997; Sokollek et al., 1998; Schüller et al, 2000; Trcek et al, 2000, 2015)
In anaerobic digestion from various floras, acetic acid bacteria play an important role in the functional niche (Wang et al, 2013)
Fermentation culture medium containing activated cell solution was used to perform the experiments of preferred cultivation conditions by changing fermentation conditions, such as the temperature, pH, ethanol concentration, carbon source, inoculum volume, and rotation speeds of the sorting table

Summary

Introduction

Acetic acid that can be produced by geological and biological processes in natural environments is an important raw material in the organic chemical industry and is the suitable carbon source for many microorganisms (Sievers et al, 1992; Trcek et al, 1997; Sokollek et al., 1998; Schüller et al, 2000; Trcek et al, 2000, 2015). The production of acetic acid using microorganisms is a promising method using low value waste as raw materials (organic waste and biomass). Anaerobic fermentation with low cost substrates rich in carbohydrates, such as organic wastes or agricultural residues, is one promising method to produce acetic acid Fermentative acidogenic microflora can produce propionic acid, butyric acid, and ethanol; acetic acid bacteria can lead them to further degrade into acetic acid and carbon dioxide or hydrogen through direct utilization of methanogenic microflora (Lange et al, 2001; Pratap et al, 2001; McHugh et al, 2003; Ge et al, 2006).

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