Abstract
Melanin is the major factor that determines skin color and protects from ultraviolet radiation. In present study we evaluated the anti-melanogenesis effect of acetazolamide (ACZ) using four different approaches: enzyme kinetic, invitro, invivo and in silico. ACZ demonstrated significant inhibitory activity (IC50 7.895±0.24μm) against tyrosinase as compared to the standard drug kojic acid (IC50 16.84±0.64μm) and kinetic analyses showed that ACZ is a non-competitive inhibitor without cytotoxic effect. In invitro experiments, A375 human melanoma cells were treated with 20 or 40μm of ACZ with or without 50μm of l-DOPA. Western blot results showed that ACZ significantly (P<0.05) decreased the expression level of tyrosinase at 40μm. Zebrafish embryos were treated with 10, 20 or 40μm of ACZ and of positive control kojic acid. At 72h of treatment with ACZ and kojic acid, ACZ significantly (P<0.001) decreased the embryos pigmentation to 40.8% of untreated embryos at the dose of 40μm of ACZ while kojic acid decreased only 25.0% of pigmentation at the same dose of kojic acid. In silico docking were performed against tyrosinase using PyRx tool. Docking studies suggested that His244, Asn260 and His85 are the major interacting residues in the binding site of the protein. In conclusion, our results suggest that ACZ is a good candidate for the inhibition of melanin and it could be used as a lead for developing the drugs for hyperpigmentary disorders and skin whitening.
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