Abstract
To study the effects of water channel protein inhibitor acetazolamide on xenograft tumor growth of colon cancer in nude mice. Setting up human colon cancer model in nude mice, mice were randomly divided into two groups as experimental group and control group. Acetazolamide was given at a volume of 0.1mL per mice (40mg/kg/d, ig) in experimental group, while the same volume of sterile saline was given in control group (ig). After 21 days, protein and m-RNA levels of AQP-1 in tumor tissues from two groups were detected respectively by Western blot and RT-PCR to evaluate the treatment effects. AQP-1, VEGF and CD34 expression was detected by immunohistochemistry, simultaneously. Acetazolamide (40mg/kg/d, ig) significantly inhibited the xenograft tumor growth of colon cancer in nude mice. The inhibition rate was 88.28%. In comparison with the control group, AQP-1 protein and mRNA level were significantly reduced in the experimental group (p<0.01). AQP-1, VEGF and CD34 expression in experimental group were positively correlated between each other (p<0.01). Acetazolamide can suppress the xenograft tumor growth by inhibiting the expression of AQP-1.
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