Acetamiprid'in Cyprinus carpio da Genotoksik Etkisinin Mikronükleus Analizi ve Comet Testi ile Araştırılması

Abstract

Pesticides are considered to be one of the biggest economic and ecological problems in the aquatic ecosystem. Monitoring for toxic effects and screening for different insecticides is vital and crucial for reducing adverse effects on aquatic organisms and public health. Therefore, in this study, we aimed to determine genotoxic effect of acetamipridine in a model fish species, Cyprinus carpio, using the micronucleus test and Comet assay. Common carp (average weight of 1.35 ±0.11g) were exposed to three different concentrations of acetamipridine (0.2, 0.4, and 0.8 g/L) based on previously detected aquatic environmental concentrations, constituting an acute test for a week. At the end of study, the Damage frequency (%), Arbitrary unit and Genetic damage index (%) were evaluated in gill and liver cells of carp by Comet assay. Also, micronucleus frequencies and erythrocyte abnormalities were determined in erythrocytes cells of carp by micronucleus test. Our results revealed significant increases in the frequencies of micronuclei and DNA strand breaks in C. carpio, following exposure to acetamipridine and thus demonstrated the genotoxic potential of this pesticide on fish. Our findings also indicated the suitability of the fish micronucleus test and comet assay in assessment of aquatic genotoxicity of insecticides.