Acetaminophen-induced alterations in pancreatic β cells and serum insulin concentrations in B6C3F1 mice

Abstract

Administration of acetaminophen (500 mg/kg) to male B6C3F1 mice resulted in alterations of pancreatic β cell ultrastructure. These alterations were characterized by pronounced intercellular spaces, cytoplasmic vacuolization, damaged membranes of cytoplasm, secretory granules, and other organelles, and pyknotic nuclei with disrupted membranes. Concomitant with these changes, acetaminophen also caused increases in serum insulin concentrations from 24 μU/ml at 0 time to 160 μU/ml at 8 hr and increases in serum alanine aminotransferase (ALT) concentrations from 42 to 13,279 U/liter, which indicated hepatic damage. Quantitation of 3-(cystein- S-yl)acetaminophen adducts in hepatic 10,000 g supernatant protein using a particle concentration fluorescence immunochemical assay indicated a positive correlation between binding and the occurrence of the hepatotoxicity consistent with that has been previously reported; however, 3-(cystein- S-yl)acetaminophen protein adducts were not detected in pancreatic 10,000 g supernatant. Immunohistochemical analysis of the liver and pancreas from acetaminophen-treated mice revealed acetaminophen-protein adducts in the centrilobular regions of the liver but not in the pancreatic islets. Doses of 100 and 200 mg/kg produced no evidence of hepatotoxicity and no increase in serum insulin; 300 mg/kg and higher doses produced both hepatotoxicity and increased serum insulin concentrations. A comparison of the time course for the increase in serum levels of ALT and insulin following a toxic dose of acetaminophen indicated that the increase in ALT preceded the increase in insulin. Thus the hepatotoxicity of acetaminophen correlates with the formation of 3-(cystein- S-yl)acetaminophen protein adducts in liver, which supports the concept that this toxicity is mediated by the reactive metabolite N-acetyl- p-benzoquinone imine; however, the toxicity of acetaminophen to β cells in the pancreas is apparently not mediated by this mechanism.