AcERF1B and AcERF073 Positively Regulate Indole-3-acetic Acid Degradation by Activating AcGH3.1 Transcription during Postharvest Kiwifruit Ripening

Abstract

Ethylene can accelerate the postharvest ripening process of kiwifruit, while indole-3-acetic acid (IAA) delays it. However, the molecular mechanism by which ethylene regulates IAA degradation is unclear. Here, we found that ethephon promotes the degradation of free IAA in kiwifruit. Furthermore, ethylene can promote the expression of AcGH3.1 and enhance its promoter activity. Two ethylene response factors (ERFs), AcERF1B and AcERF073, were obtained using an AcGH3.1 promoter as bait for a yeast one-hybrid screening library. Both AcERF1B and AcERF073 bind to the AcGH3.1 promoter to activate it. Also, AcERF1B/073 enhanced AcGH3.1 expression, decreased the free IAA content, and increased the IAA-Asp content in kiwifruit. In addition, we found that the AcERF1B and AcERF073 proteins directly interact, and this interaction enhanced their binding to the AcGH3.1 promoter. In summary, our results suggest that AcERF1B and AcERF073 positively regulate IAA degradation by activating AcGH3.1 transcription, which accelerated postharvest kiwifruit ripening.