Accuracy of quantitative real-time PCR analysis

Abstract

When solving many problems in biology and med� icine, it is necessary to detect the presence of specific nucleic acid sequences in samples. For this purpose, polymerase chain reaction (PCR)—a chain enzy� matic reaction of multiplication of the analyzed DNA fragment, realized in the form of successive rounds of duplication of fragments present in the reaction—is commonly used. In addition to identification of a specific DNA sequence, it is often necessary to determine its con� centration in the sample. At present, the most com� mon method for quantitative analysis of nucleic acids