Abstract
Stable lines of both callus and cell suspensions of Salvia sclarea and callus of Nicotiana glutinosa accumulated sclareol at rates (μg g−1 day −1) varying from 0.2 to 6% of those found in the parent plants. Accumulations of the anti-fungal diterpene were at a maximum near entry to the exponential growth phase. Despite these apparent relative biosynthetic deficiencies, callus of both species incorporated 3R-[2-4C]mevalonate into the compound with efficiencies ca 30% of those displayed by the parent plants under similar external environments.
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