Abstract
The mRNA accumulation of a number of different genes during different stages of almond fruit development has been studied. The probes used in the study correspond to almond cDNAs previously characterized in our laboratory. They include cDNAs encoding extensin, prunin (the main storage protein in almond seed), oleosin, α-tubulin and a cDNA clone (PA3BF11) not showing any similarity to known sequences but whose mRNA is very abundant in the almond seed. These probes have been used in RNA blot, tissue printing and in situ hybridization experiments. The tissue printing technique has proven to be a very useful tool for detecting gene expression in large organs such as whole almond fruits whereas in situ hybridization can be successfully applied in small fruits. It is shown that the distribution of specific mRNAs is highly controlled in the fruit. mRNAs related to storage proteins are abundant in seed tissues while mRNAs corresponding to genes related to cell division, such as extensin or α-tubulin, are accumulated in dividing cells mostly around the vascular tissues.
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