Abstract
Selenium is considered an essential component of all living organisms. Studies on the enrichment of yeast cells with selenium, using the ability of cell biomass to bind this element, are being reported more and more. Yeast cultures were cultivated in YPD medium enriched with Na2SeO3 salts for 72 h at 28 °C on a shaker utilizing reciprocating motion. Selenium in cell biomass was determined with the use of ICP–MS. It was observed that the addition of selenium to the experimental medium (in the range of 4–100 mg/L) increased the content of this element in the yeast cell biomass. During the extension of cultivation time, the number of yeast cells and biomass yield exhibited a decreasing trend. Based on the obtained results, it was concluded that yeast cells exhibited the ability to accumulate selenium in both logarithmic and stationary growth phases. The dose of 20 and 30 mg/L of selenium in the culture medium meets the expectations in terms of both the content of selenium bound to yeast cells (1944 ± 110.8 μg/g dry weight) under 48-h cultivation. The obtained results confirmed that the Candida utilis ATCC 9950 strain exhibits the ability to bind selenium, which means that the biomass of these yeasts may be used as a natural source of selenium in the diet of humans and animals.
Highlights
Selenium is all an essential mineral for the proper functioning of cellular and body metabolism [1].Selenium is a component of many enzymes, and some proteins
The objective of this study was to evaluate the possibility of intracellular binding of selenium by Candida utilis ATCC 9950 yeast in media supplemented with selenium in the range of 4–100 mg/L, and to determine the content of protein in yeast biomass
Results obtained by Nam et al [26] showed that the addition of selenium to the YM (Yeast Mold) culture medium (24 h) at a concentration between 30 and 125 mg/L led to an increase in the content of selenium in the cell biomass of Saccharomyces cerevisiae 6 M in the range between 1039 and 5003 μg/g
Summary
Selenium is a component of many enzymes, and some proteins. It has an enzymatic and stabilizing function. Stimulates the immune system and exhibits antiproliferative activity [2,3,4]. Selenium plays a role in optimizing thyroid function [5]. Selenium is an element found in many enzymes, especially those with antioxidant activity: glutathione peroxidase (GPx), iodothyronine deiodinase (DIO) and thioredoxin reductase (TRxR). These enzymes prevent the formation of hydrogen peroxide and the peroxidation of phospholipid cell membranes [6,7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
