Accumulation of epoxiconazole from soil via oleic acid-embedded cellulose acetate membranes and bioavailability evaluation in earthworms (Eisenia fetida)

Abstract

A passive sampler in the soil environment is a relatively novel technique and has had quite limited applications, especially for pesticides. Oleic acid-embedded cellulose acetate membranes (OECAMs) were developed to evaluate the bioavailability of epoxiconazole (EPO) to earthworms (Eisenia fetida). The uptake of EPO by OECAMs (R2 = 0.975) and earthworms (R2 = 0.938) was compared and found to follow a two-compartment kinetic model. EPO sampling by OECAMs reached equilibrium (94%) within 2 d. OECAM could be used to determine the concentration of EPO in soil porewater. Furthermore, a significant linear relationship (R2 = 0.990) was observed between the EPO concentrations in earthworms and the OECAMs. The EPO concentrations in the porewater and OECAMs were lower in soils with a higher organic matter (OM) content. The EPO concentrations in the porewater, earthworms, and OECAMs decreased by 64.4, 49.0, and 56.1%, respectively, in the presence of 0.5% biochar, compared with the control. Furthermore, the use of OECAMs versus earthworms for soil testing also allows you to avoid factors that increase variance in organisms, such as avoidance behaviors or feeding. Therefore, OECAMs show good potential for use as a passive sampler to evaluate the bioavailability of EPO.