Accumulation of CD4+CD28-PD-1lowOX40+ T cells and its clinical significance in rheumatoid and collagen-induced arthritis

Abstract

Event Abstract Back to Event Accumulation of CD4+CD28-PD-1lowOX40+ T cells and its clinical significance in rheumatoid and collagen-induced arthritis Juean Jiang1, Cuiping Liu1, Yongjing Chen2, Jianhua Jiang1, Ranran Zhu2, Ting Wang2, Min Wu3 and Xueguang Zhang1* 1 The First Affiliated Hospital of Soochow University, China 2 Soochow University, China 3 The Third Affiliated Hospital of Soochow University, China CD4+CD28- T cells play a key role in the immunopathogenesis of rheumatoid arthritis (RA). Co-inhibitory molecule of PD-1 has a crucial role in suppression of T cell responses. However, presence of PD-1 and absence of CD28 do not induce CD4+ T cells into apoptosis in RA. In this study, we characterized a novel CD4+CD28-PD-1lowOX40+ T cells subset and investigated its clinical significance in rheumatoid and collagen-induced arthritis (CIA). Peripheral blood samples were collected from RA, osteoarthritis and healthy subjects. CIA was induced in DBA/1 mice. After separation of mononuclear cells from peripheral blood of the patients or spleen of CIA mice, CD4+CD28-PD-1lowOX40+ T cells were examined by flow cytometry. In RA patients and CIA mice, there were increased expressions of PD-1 and OX40 on CD4+ T cells, with PD-L1 and OX40L expressions augmented on monocytes and B cells. Analyses showed that CD4+CD28-PD-1lowOX40+ T cells were accumulated and closely correlated with clinicopathological features of RA patients and CIA mice. Moreover, immunosuppressive therapy significantly reduced the percentage of CD4+CD28-PD-1lowOX40+ T cells. Additionally, CD4+CD28-PD-1low T cells displayed a phenotype of CD45RAlowCD45ROhighCD25low. In summary, low PD-1 and enhanced OX40 expression implied longevity and persistent activation of these T cells both in RA patients and CIA mice. A phenotype of memory T cells and fine correlations with clinicopathological features suggested that these T cells may be involved in the immunopathogenesis of RA and CIA. Interventions with pathological functions of this T cells subset may provide a new therapeutic interesting in RA. Acknowledgements This study was supported by the National Natural Science Foundation of China (Grants: 30930085, 81001337 and 31170834), the Research and Innovation Project for College Graduates of Jiangsu Province (Grant: CXLX11_0077), and Science and Technology Support Program of Suzhou City (Grant: SS201246). Keywords: Rheumatoid arthritis, Autoreactive T cells, CD28, PD-1, OX40 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Jiang J, Liu C, Chen Y, Jiang J, Zhu R, Wang T, Wu M and Zhang X (2013). Accumulation of CD4+CD28-PD-1lowOX40+ T cells and its clinical significance in rheumatoid and collagen-induced arthritis. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00742 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Xueguang Zhang, The First Affiliated Hospital of Soochow University, Suzhou, China, xueguangzh@126.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Juean Jiang Cuiping Liu Yongjing Chen Jianhua Jiang Ranran Zhu Ting Wang Min Wu Xueguang Zhang Google Juean Jiang Cuiping Liu Yongjing Chen Jianhua Jiang Ranran Zhu Ting Wang Min Wu Xueguang Zhang Google Scholar Juean Jiang Cuiping Liu Yongjing Chen Jianhua Jiang Ranran Zhu Ting Wang Min Wu Xueguang Zhang PubMed Juean Jiang Cuiping Liu Yongjing Chen Jianhua Jiang Ranran Zhu Ting Wang Min Wu Xueguang Zhang Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.