Abstract
In Limnanthes alba seeds, an accumulation of phosphatidic acid (PA) occurred during seed development until the day 15 after pollination (DAP). Between the 15 and the 20 DAP, a rapid synthesis of triacylglycerols (TG) took place with a simultaneous decrease of PA. During the same period, very long-chain fatty acids (VLCFA), viz. 20: 1 Δ 5, 22:1 Δ 13 and 22:2Δ 5,13 were synthesized and rapidly channelled into TG. These results suggest the involvement of glycerol-3-phosphate in TG biosynthesis. Moreover, TG synthesis appears to be a time-compartmentalized event; the first accumulation of PA having short-chain acyl moieties then, from the 15 DAP, synthesis of VLCFA which are esterified in TG originating from the PA accumulated during the first period.
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