Abstract
D,L-Homocysteine, at the concentration of 10 mM, inhibited the methylation reaction of sterol side chain in cell-free extract of yeast, but did not inhibit 14C-incorporation from [ 14C]mevalonate into nonsaponifiable lipids. Under this condition, a radioactive C 27-sterol was accumulated. Examination by gas-chromatography on a DEGS column, partial hydrogenation, side chain cleavage, and by methylation with crude methyl transferase preparation, suggested the accumulated sterol to be 5α-cholesta-7, 24-diene-3 β-ol. The possible role of this sterol as a natural acceptor of the methyl group in ergosterol biosynthesis of yeast was discussed.
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