Accumulation of 1,3-beta-D-glucans, in response to aluminum and cytosolic calcium in Triticum aestivum.

Abstract

One of the most rapid responses to aluminum (Al) stress in plants is enhanced synthesis and deposition of 1,3-beta-D-glucans (callose) in root tips. Ironically, Al-induced synthesis and deposition of callose occurs in vivo, despite evidence from in vitro systems that suggests that Al is a powerful inhibitor of 1,3-beta-D-glucan synthase. We set out to test the hypothesis that an Al-induced increase in the activity of free calcium in the cytoplasm ([Ca(2+)](cyt)) is the trigger for enhanced synthesis of callose in in vivo systems, an effect that would not be observed in in vitro systems. Root tips of an Al-sensitive cultivar of Triticum aestivum were treated with Al (0-100 microM) or the Ca ionophore A23187 (0-3 micro M) for 3-24 h, and the effects on [Ca(2+)](cyt) and synthesis of callose were measured using confocal laser scanning microscopy. Treatment with Al induced a rapid increase in both [Ca(2+)](cyt) (4.7-fold) and synthesis of callose (30-fold). Treatment with the Ca ionophore, A23187, also elicited an increase in [Ca(2+)](cyt) (6.6-fold). Despite a greater increase in [Ca(2+)](cyt) in the presence of A23187, this increase was accompanied by a smaller increase in callose deposition (11-fold) than was observed in the presence of Al. These data suggest that an increase in [Ca(2+)](cyt) is not the only factor modulating increases in callose synthesis and deposition in the presence of Al.