Accumulation and esterification of diarrhetic shellfish toxins from the aqueous phase in laboratory-exposed mussels

Abstract

In recent years, marine bivalves cultured in the natural environment have been confirmed to accumulate diarrhetic shellfish toxins (DSTs) from the aqueous phase. To investigate the effects of varying seston concentrations on DST accumulation, mussels (Mytilus galloprovincialis) were exposed to comparable concentrations of okadaic acid (OA) and dinophysistoxin-1 (DTX1) in 0.45-μm filtered seawater spiked with varying concentrations of ambient suspended particles at 0, 10, 30, 60, 90, and 120 mg L−1, for 96 h. Effects of seston additions on the mussels’ feeding on nontoxic microalgae, Isochrysis galbana, the stability of dissolved toxins and the anatomical compartmentalization of toxins were also assessed. Results showed that mussels more readily accumulated OA than DTX1 from the aqueous phase. Three potential mechanisms of the effects of seston on toxin accumulation were identified. First, seston at low concentrations (10 mg L−1) adsorbed toxins and thus promoted toxin accumulation. Second, seston enhanced the degradation of aqueous OA and DTX1, and possibly reduced the adsorption by digestive gland (DG) cells through simple diffusion due to competitive adsorption. Third, the clearance rate of mussels was significantly reduced at high seston concentrations (120 mg L−1). The esterification of DSTs was maximized in DG tissue, although a high percentage (52%) of DSTs was distributed in non-visceral tissues of mussels exposed to aqueous toxins. This study suggests that the risk of benthic DST-producing microalgae to marine cultured shellfish should be taken into consideration, even in the absence of a bloom of toxic microalgae in the water column.