Accumulation and Distribution of Hydrogen Peroxide in Interaction between Sugarbeet Plant and Sugarbeet Necrotic Yellow Vein Virus

Abstract

Hydrogen peroxide(H_2O_2)plays an important role in the interaction between plant and pathogen defense response.The objective of this research was owing the interaction system of beet necrotic yellow vein virus(BNYVV)and resistant,susceptible sugarbeet lines to disclose the relationship of accumulation and distribution of H_2O_2 with sugarbeet rhizomania resistance.The production and accumulation of H_2O_2 in the two interaction systems were detected and the distribution of H_2O_2 was observed in subcellular level using the cytochemical technique.The result showed that there was plenty of H_2O_2 in both interaction systems,and the production of H_2O_2 was evidently higher in the resistant lines than with in the susceptible lines.In addition,distribution and the location of H_2O_2 were similar in the two lines,where we observed mainly in the tonoplast and the plasma membrane of host root,vein cells as well as the intercellular space of some host vein cells.But there were obvious differences in the content of H_2O_2 accumulated in the two interaction lines,the deposition of H_2O_2 in the susceptible line was significantly lower than that in the resistant line,showing it may have a close relationship with resistance of sugarbeet against rhizomania,H_2O_2 showed the involved in the defense responses to virus infection in sugar beets.