Accumulated forms of thallium and cadmium inLemna minor. II. Relationship between duration of exposure and metal-protein binding

Abstract

AbstractGel filtration chromatography (with Sephadex G25, G50 and G100) was used to separate the different forms of thallium (Tl) and cadmium (Cd) in the cytosol fraction of Lemna minor and to examine the influence of the duration of metal exposure on the speciation of the two elements. A major proportion of Cd in the soluble phase was found to be bound to three groups of proteinaceous and polypeptide fractions; two of these, the high molecular weight protein fraction (Mr > 150,000) and the low polypeptide sized moieties of about Mr 1,500 or less were constitutive entities, whereas the intermediate sized fraction (Mr 7,000 - 8,000) could only be detected in plants previously exposed to Cd. After 12 days of exposure to Cd this fraction accounted for the greatest part of the bound Cd in Lemna tissues. Extending the period of exposure from 18 hours to 12 days resulted in a shift in the distribution of Cd between the low and intermediate fractions. Evidence for Tl-protein binding was limited and confined to t...