Acclimation to partial shading or full sunlight determines the performance of container-grown Fraxinus ornus to subsequent drought stress

Abstract

Cultivation techniques in the nursery aimed to improve tolerance to abiotic stresses after planting in the landscape may have great ecological value. Here we tested the hypothesis that the irradiance at which plants grow in the nursery may impact on their responses to a subsequent drought stress. Measurements of relevant morpho-anatomical, physiological and biochemical traits were conducted in Fraxinus ornus, a member of the Oleaceae family of increasing interest as an urban tree. Leaves developed under moderate shade displayed a lower ability to preserve water balance, thus suffering from a greater decline in net CO2 assimilation than leaves acclimated to full sunlight, when subsequently exposed to drought stress. Shaded leaves also displayed an inherently lower capacity to dissipate excess radiant energy through nonphotochemical quenching, thus suffering from greater oxidative damage than sun leaves under drought. These results indicate that sunlight irradiance at which container-grown ash trees grow in the nursery may tightly affect the tolerance to subsequent drought stress. This may have great value for enhancing the establishment of container-grown plants in the landscape, as fully leaved plants may suffer from severe drought stress during early stages of acclimation in the landscape.