Acclimation of Stomatal Conductance to a CO2-Enriched Atmosphere and Elevated Temperature in Chenopodium album

Abstract

Acclimation of stomatal conductance to different CO2 and temperature regimes was determined in Chenopodium album L. plants grown at one of three treatment conditions: 23�C and 350 μmol CO2 mol-1 air; 34�C and 350 μmol mol-1; and 34�C and 750 μmol mol-1. Stomatal conductance (gs) as a function of intercellular CO2 (Ci) was determined for each treatment at 25 and 35�C, and these data were used to estimate gains of the feedback loops linking changes in intercellular CO2 with stomatal conductance and net CO2 assimilation. Growth temperature affected the sensitivity of stomata to measurement temperature in a pattern that was influenced by intercellular CO2. Stomatal conductance more than doubled at intercellular CO2 varying between 200 and 600 μmol mol-1 as leaf temperature increased from 25 to 35�C for plants grown at 23�C. In contrast, stomatal conductance was almost unaffected by measurement temperature in plants grown at 34�C. Elevated growth CO2 attenuated the response of stomatal conductance to CO2, but growth temperature did not. Stomatal sensitivity to Ci was extended to higher Ci in plants grown in elevated CO2. As a result, plants grown at 750 μmol mol-1 CO2 had higher Ci/Ca at ambient CO2 values between 300 and 1200 �mol mol-1 than plants grown at 350 �mol mol-1 CO2. The gain of the stomatal loop was reduced in plants grown at elevated CO2 or at lower temperature when compared to plants grown at 350 μmol mol-1 and 34°C. Both photosynthetic and stomatal loop gains acclimated to elevated CO2 in proportion so that their ratio, integrated over the range of Ci in which the plant operates, remained constant. Water use efficiency (WUE) more than doubled after a short-term doubling of ambient CO2. However, the WUE of plant grown and measured at elevated CO2 was only about 1.5 times that of plant transiently exposed to elevated CO2, due to stomatal acclimation. An optimal strategy of water use was maintained for all growth treatments.