Abstract
This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A 620/A 280 ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A 620/A 280 ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.
Highlights
Microalgae are a diverse group of photosynthetic microorganisms that can grow in a wide range of habitats (Khan et al, 2018)
To evaluate the effect of different temperatures on two native cyanobacterial species, the cultures were incubated at 80 μmol photons m−2 s−1 and the temperatures of 24, 28, 32, and 36 ± 1°C, whereas in the light intensity trial, cultures were exposed to different photon flux densities of 40, 60, 80, 100, 120, 150, and 200 μmol photons m−2 s−1, and the temperature of 28 ± 1 and 32 ± 1°C for the strains CF1 and CF3, respectively
The second cyanobacteria isolate showed resemblance with the Oscillatoriaceae family on the basis of its morphological characters, which was further confirmed as Desertifilum sp. by 16S rRNA gene sequencing
Summary
Microalgae are a diverse group of photosynthetic microorganisms that can grow in a wide range of habitats (Khan et al, 2018). They have a high growth rate, simple growth requirements, and can efficiently accumulate large quantities of proteins, lipids, carbohydrates, and carotenoids (Hsieh-Lo et al, 2019). Cyanobacteria are amazing living factories that can be customized for the production of valuable products. Their biomass may be converted to various value-added products such as biofuels, dietary supplements, feed, and cosmetics (Ho et al, 2018; Ma et al, 2020). The cyanobacteria species are potential producers of useful secondary metabolites including xanthophylls and phycobiliprotein (Rastogi and Sinha, 2009; Khatoon et al, 2018)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
