Accessory cell function of human alveolar macrophages in B-cell activation induced by pokeweed mitogen

Abstract

The effect of alveolar macrophages (AM) on pokeweed mitogen (PWM)-stimulated immunoglobulin (Ig) secretion by unfractionated and monocyte-depleted human peripheral blood mononuclear cells was studied. Responsiveness in monocyte-depleted peripheral blood mononuclear cell populations could be partially restored by addition of autologous monocytes and to a lesser extent with AM. Addition of AM to unfractionated peripheral blood mononuclear cells resulted in significant inhibition of Ig secretion, especially at high (5–10 μg/ml) doses of PWM. The degree of suppression was proportional to the number of AM present. On the other hand, addition of monocytes to similar unfractionated peripheral blood mononuclear cell cultures did not result in suppression of Ig secretion at any of the doses of PWM used. Suppression by AM was not attributable to an alteration of response kinetics. The results demonstrate that mononuclear phagocytic cells are necessary for activation of polyclonal Ig secretion by human B cells and that AM are capable of suppressing this response.